Metastatic breast and colon cancer regulated genes

An isolated and purified subgenomic polynucleotide comprising a nucleotide segment selected from the group consisting of:

• (a) a segment of at least 30 contiguous nucleotides which hybridizes under stringent conditions to a nucleotide sequence selected from the group consisting of SEQ ID NO:2, 3, 6, 7, 8, 12, and 13;
• and
• (b) a segment of at least 75 contiguous nucleotides which hybridizes under stringent conditions to a nucleotide sequence of SEQ ID NO:9;
• wherein said subgenomic polynucleotide is expressed at a higher level in non-metastatic breast cancer tissue relative to metastatic breast cancer tissue, wherein said stringent conditions are selected from the group consisting of 4×SSC at 65° C.;
• 50% formamide, 4×SSC at 42° C.;
• or 0.5×SSC, 0.1% SDS at 65° C.

A host cell comprising a construct which comprises:

• a promoter;
• and
• a polynucleotide segment comprising a nucleotide sequence selected from the group consisting of SEQ ID NOS:
• 2, 3, 5, 6, 7, 8, 9, 12, 13 and 14.

A recombinant host cell comprising a transcription initiation unit wherein the transcription initiation unit comprises in 5′ to 3′ order:

• (a) an exogenous regulatory sequence;
• (b) an exogenous exon;
• and
• (c) a splice donor site;
• wherein the transcription initiation unit is located upstream of a coding sequence of a gene, wherein the coding sequence comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS:2, 3, 5, 6, 7, 8, 9, 12, 13 and 14, wherein the exogenous regulatory sequence controls transcription of the coding sequence of the gene.