Chlamydial vaccines and immunogenic compositions containing an outer membrane antigen and methods of preparation thereof
AssigneeAventis Pasteur Limited
InventorsAndrew D. Murdin, Brian J. Underdown
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Abstract
Immunogenic compositions including vaccines are described that comprise an outer membrane antigen extract of a strain of Chlamydia and are effective in protection against disease caused by Chlamydia infection The immunogenic compositions may comprise the major outer membrane protein (MOMP) of Chlamydia which may be in a homooligomeric form or complexed with at least one other antigen of Chlamydia. The immunogenic composition may include an immunostimulating complex (ISCOM) and the outer membrane antigen may be incorporated therein. The immunogenic compositions have utility as chlamydial vaccines and in diagnostic applications.
Claims5
A method of producing an outer membrane antigen extract of a strain of Chlamydia, which comprises:
- detergent extracting elementary bodies of said strain of Chlamydia in the presence of a reducing agent to solubilize cytoplasmic material away from outer membrane material;
- separating said solubilized cytoplasmic material from the outer membrane materials;
- detergent extracting said outer membrane material using at least two non-ionic detergents in the presence of a reducing agent to solubilize outer membrane antigens;
- and
- separating said solubilized outer membrane antigens from residual unextracted membrane-associated material to provide said outer membrane antigen extract.
Dependencies are inferred from the claim text.