Back to results
US 6,635,745 B2Granted
Rapamycin assay
AssigneeNovartis AG
InventorsRichard Sedrani, Valérie Quesniaux Ryffel
Life of this patent
Estimating the term…
Abstract
Monoclonal antibodies to rapamycin and to 40-O-alkylated derivatives of rapamycin are provided, together with novel haptens, immunogenic conjugates, and processes for making them and assay kits for using them.
Claims4
An immunogenic conjugate which is 1) rapamycin bearing an immunogenic protein at position 40 or 28;
- or 2) 40-O-(2-hydroxyethyl)-rapamycin bearing an immunogenic protein at position 28.
An immunogenic conjugate comprising a rapamycin portion and a protein portion wherein the rapamycin is
- i) 40-O-succinimidooxysuccinyl-rapamycin,
- ii) 28-O-succinimidooxysuccinyl-rapamycin, or
- iii) 28-O-(succinimidooxysuccinyl)-40-O-(2-hydroxyethyl)-rapamycin.
An immunogenic conjugate which is produced by reacting a protein with a rapamycin derivative bearing an activated coupling group wherein the rapamycin is
- i) 40-O-succinimidooxysuccinyl-rapamycin,
- ii) 28-O-succinimidooxysuccinyl-rapamycin, or
- iii) 28-O-(succinimidooxysuccinyl)-40-O-(2-hydroxyethyl)-rapamycin.
Dependencies are inferred from the claim text.