Lipoproteins as nucleic acid vectors
AssigneeBaylor College of Medicine
InventorsRon C. Hoogeveen, J. Paul Moore
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Abstract
The present invention relates to materials and methods for the in vivo transport and deliver of nucleic acids. More particularly, it concerns the use of lipoproteins, including but not limited to, low density lipoproteins (“LDL”), and/or apolipoproteins for the binding and in vivo transport of nucleic acids. In addition, the present invention relates to the use of lipoproteins in the early detection of cancer and/or metastatic cancer and/or arteriosclerosis.
Claims33
A method for expressing a selected polypeptide in a human cell comprising:
- a) providing a composition comprising (i) a polypeptide comprising a non-murine, native low density lipoprotein (LDL) and (ii) a nucleic acid comprising an expression cassette encoding said selected polypeptide, wherein said coding sequence is operably linked to a promoter, and wherein said nucleic acid is bound to said LDL;
- b) delivering said composition to said cell under conditions permitting transfer of said composition into said cell;
- and
- c) culturing said cell under conditions permitting the expression of said selected polypeptide.
A method for providing an expression construct to a human cell comprising:
- a) providing a composition comprising (i) a polypeptide comprising a non-murine, native low density lipoprotein (LDL) and (ii) a nucleic acid comprising an expression cassette, encoding an expression region and a promoter active in eukaryotic cells, wherein said expression region is operably linked to said promoter, and wherein said nucleic acid sequence is bound to said LDL;
- and
- b) delivering said composition to said cell under conditions permitting transfer of said composition into said cell.
A method inhibiting the growth of a tumor in vivo comprising:
- a) providing a composition comprising (i) a polypeptide comprising non-murine native low density lipoprotein (LDL) and (ii) a nucleic acid construct comprising an expression cassette encoding an anti-tumor expressible product and a promoter active in eukaryotic cells, wherein said nucleic acid construct is bound to said LDL;
- and
- b) delivering said composition to a human subject by local administration to said tumor under conditions permitting transfer of said composition into cells of said tumor,
- whereby said tumor is inhibited by said expressible product.
Dependencies are inferred from the claim text.