A method for the detection of an analyte in a sample, the method comprising the steps of
- a) contacting the sample with a device, which device comprises a substrate having through-going channels, said channels opening out on a surface for sample application, the channels in at least one area of the surface for sample application being provided with a first binding substance capable of binding to an analyte, wherein the substrate is an electrochemically manufactured metal oxide membrane and the first binding substance is within the through-going channels in the substrate;
- b) passing the sample through the membrane in one direction perpendicular to the surface of the membrane in a manner sufficient to allow binding to take place between the first binding substance and the analyte to be detected;
- c) repeating steps a) and b) by passing the sample through the membrane in the opposite direction;
- and
- d) detecting whether binding has occurred between the first binding substance and the analyte.