Procedure for the simultaneous quantitative and qualitative analysis of both flavonoid glycosides and steroidal glycosides
AssigneeNoble Laboratories
InventorsKejian Fu, Maciej A. Kaminski, Jessica Liu
Life of this patent
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Abstract
A single rapid procedure for the quantitative and qualitative analysis of flavonoid glycosides and steroidal glycosides, in natural products, has been developed. The material is extracted with aqueous solutions of polar organic solvents in an ultrasonic bath. Interfering compounds are removed by pre-purification procedures prior to instrumental analysis by High Pressure Liquid Chromatography with Negative Ion Electrospray Mass Spectrometric Detection and on-line Diode Array Ultraviolet-Visible Spectroscopy. The analysis of flavonoid and steroidal glycosidic compounds in HOSTA leaves served as a model system.
Claims30
A method of analytically determining and characterizing Hosta flavonoid glycosides and Hosta steroidal glycosides comprising the following steps:
- (a) obtaining a sample to be analyzed;
- (b) preparing said sample to be analyzed;
- (c) mixing said sample with aqueous polar solvents to make a crude extract;
- (d) pre-purifying said crude extract to remove interfering components;
- (e) isolating said Hosta flavonoid glycosides and Hosta steroidal glycosides through actively guided fractionation;
- and
- (f) analyzing and simultaneously identifying said Hosta flavonoid glycosides and Hosta steroidal glycosides by use of High Pressure Liquid Chromatography (HPLC) combined with Electrospray Ionization Mass Spectrometry and Diode Array Ultra-Violet and Visible Spectroscopy detection techniques.
A method of analytically determining and characterizing Hosta flavonoid glycosides and Hosta steroidal glycosides comprising the following steps:
- a) obtaining a sample to be analyzed;
- b) preparing said sample to be analyzed;
- c) mixing said sample with aqueous polar solvents to make a crude extract;
- d) pre-purifying said crude extract to remove interfering components;
- e) isolating said Hosta flavonoid glycosides and Hosta steroidal glycosides through actively guided fractionation;
- and
- f) analyzing and identifying said Hosta flavonoid glycosides and Hosta steroidal glycosides.
Dependencies are inferred from the claim text.