Stabilization of calibrators containing cytokeratin
AssigneeRoche Diagnostics GmbH
InventorsApostolos Kyriatsoulis, Gunter Pappert, Ellen Moessner, Norbert Franken, Michael Rottmann, Heinz Bodenmueller
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Abstract
The invention concerns calibrators or calibration solutions which are based on a human serum matrix and which are used in a method for detecting cytokeratin, a process for producing them, a method for stabilizing cytokeratin and an immunological method for detecting cytokeratin in a sample.
Claims24
A calibrator for use in the detection of cytokeratins, the calibrator comprising:
- a serum, a known concentration of cytokeratin, and aprotinin.
A method for stabilizing a cytokeratin calibrator wherein said calibrator contains a serum and a known concentration of cytokeratin, said method comprising adding aprotinin to the calibrator.
A process for producing a cytokeratin calibrator, the process comprising:
- mixing a serum with aprotinin to form a solution;
- dissolving a known concentration of cytokeratin in water;
- mixing said solution and said dissolved cytokeratin to form the cytokeratin calibrator.
A method for determining the concentration of cytokeratin in a sample, the method comprising:
- measuring a first value relating to the concentration of cytokeratin in the sample;
- measuring a second value relating to the concentration of cytokeratin in a calibrator using the same methodology used to measure the first value, the calibrator comprising a serum, a known concentration of cytokeratin and aprotinin;
- and
- comparing the measured first value to the measured second value to determine the concentration of cytokeratin in the sample.
Dependencies are inferred from the claim text.