Targeted vectors
AssigneeCanji, Inc.
InventorRichard B. Murphy
Life of this patent
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Abstract
This invention provides therapeutic and diagnostic agent delivery vehicles, including viral vectors, that are complexed to a targeting moiety by coordinate covalent linkages mediated by a transition metal ion. The complex is typically formed with a transition metal ion that is in a kinetically labile oxidation state; after the complex is formed, the oxidation state of the transition metal ion is changed to one that renders the complex kinetically stable. The use of a coordinate covalent linkage to attach the targeting moiety to the delivery vehicle provides the ability to readily attach a different targeting moiety to a delivery vehicle without modifying the delivery vehicle itself. This flexibility is achieved without sacrificing stability of the complex.
Claims26
A targeted complex of the formula:
- {{recombinant virus-CM}-TMI-{CM-targeting ligand}};
- wherein CM is a chelating peptide, TMI is a transition metal ion, and CM-targeting ligand is a chelating peptide (CM) covalently linked to a targeting ligand.
A viral vector complex that comprises a targeting ligand that is attached to a surface polypeptide of a viral vector by a coordinate covalent linkage mediated by a transition metal ion.
A method of producing a kinetically inert targeted recombinant virus complex, the method comprising:
- a) preparing a kinetically labile transition metal complex by contacting recombinant virus-CM and a CM-targeting ligand with a transition metal ion that is in a kinetically labile oxidation state;
- and
- b) changing the oxidation state of the metal ion to form the kinetically inert complex.
Dependencies are inferred from the claim text.