Retrovirus and viral vectors
AssigneeRubicon Laboratory, Inc.
InventorVit Lauermann
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Abstract
This invention relates to the fields of genetic engineering, virus replication and gene transfer. More specifically, this invention relates to polynucleotide construct, recombinant virus, transposon, and their vectors, wherein an ori derived from a DNA virus capable of replicating in vertebrate cells is inserted into the retrovirus, allowing the retrovirus following the reverse transcription to efficiently replicate as extrachromosomal or episomal DNA without the necessity of integration into the host cell chromosome. Additionally, this invention relates to polynucleotide construct, recombinant virus, transposon, and their vectors replicating episomally without aid of an ori and related elements. Also, this invention encompasses preventive, therapeutic, and diagnostic applications employing said constructs, viruses and vectors.
Claims24
A polynucleotide construct comprising retroviral sequence encoding at least one LTR, polypurine tract and packaging signal of a retroviral genome of an episomally replicating retrovirus, which is able to replicate without requirement of integration, said retroviral sequences further comprising one or more mutations that disable the integration of said construct into host chromosomal DNA, said construct further having the capacity to replicate via reverse transcription, provided that any reverse transcription product obtained from such reverse transcription is also disabled from integrating into host chromosomal DNA, said retroviral sequence further comprising a heterologous sequence encoding a gene product of interest.
A composition comprising retroviral sequence encoding all the genetic elements necessary for the production of an immunogenic virion, including one or more LTRs, said genetic elements including one or more mutations that disable the integration of viral DNA into host chromosomal DNA, such that any DNA molecules arising from a reverse transcription step involving an RNA of said immunogenic virion are able to exist episomally within host vertebrate cells, said virion further being able to replicate without requirement of integration, said retroviral sequence further comprising a heterologous sequence encoding a gene product of interest.
Dependencies are inferred from the claim text.