Selection for more efficient transformation host cells
AssigneeStratagene
InventorAlan L. Greener
Life of this patent
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Abstract
This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and selecting host cells that survive the transformation conditions. This invention also provides methods for transferring nucleic acids of interest into host cells, using cells that are better able to survive transformation treatment. Also, this invention provides kits for producing or selecting host cells in transformation treatments, as well as, kits comprising various host cells that may be utilized in transformation experiments.
Claims76
A method for producing an altered cell line that better survives transformation treatment than unaltered host cells, comprising:
- (a) subjecting host cells to conditions that alter at least a portion of the host cells, wherein the conditions that alter the hosts cells are mutation conditions that mutate at least one of the host cells;
- (b) subjecting the altered host cells of (a) to transformation conditions;
- (c) selecting altered host cells of (b) that survive the transformation conditions;
- and
- (d) growing the selected host cells from (c) to generate an altered cell line that better survives transformation treatment than unaltered host cells.
A method for producing an altered cell line that better survives transformation treatment than unaltered host cells, comprising:
- (a) obtaining host cells that have been altered, wherein the hosts cells have been altered by mutation conditions that mutate at least one of the host cells;
- (b) subjecting the altered host cells of (a) to transformation conditions;
- (c) selecting altered host cells of (b) that survive the transformation conditions;
- and
- (d) growing the selected host cells from (c) to generate an altered cell line that better survives transformation treatment than unaltered host cells.
A bacterial strain designated XL1-Blue MRF′ B150.
A method for producing a cell line that better survives transformation treatment that unselected host cells, comprising:
- (a) subjecting host cells to transformation conditions;
- (b) selecting host cells of (a) that survive the transformation conditions;
- and
- (c) growing the selected host cells from (b) to generate a cell line that better survives transformation treatment than unselected host cells.
The method of 43, further comprising culturing the cells transformed in (c) in a selected media capable of promoting their growth.
The method of 43, wherein cells are prokaryotic or eukaryotic.
The method of 56, further comprising culturing the cells transformed in (c) in a selected media capable of promoting their growth.
The method of 56, wherein cells are prokaryotic or eukaryotic.
Dependencies are inferred from the claim text.