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US 6,635,456 B2Granted
Procedure for the preparation of pure phosphatides with phospholipase D
AssigneeFidia Farmaceutici S.P.A.
InventorsGuenter Kirschner, Giampaolo Menon, Susanna Vaccaro
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Abstract
Process for preparing pure phosphatides starting from mixtures of natural phosphatides, or their single components, such as soybean or egg lecithin or animal phospholipids, or from synthetic phosphatides by reacting them both with phospholipase D derived from Streptomyces hachijoense in a completely aqueous medium in the presence of defined substates containing a primary or secondary alcoholic group, and their uses thereof.
Claims10
A process for the preparation of phosphatides with the formula (I):
- R—O—PO(OH)—O—R1 (I)
- wherein R is diacylglycerol and R1 is an hydroxl group which process comprises:
- reacting a phosphatide of the formula (II):
- R—O—PO(OH)—O—R2 (II)
- wherein R means as above and R2 is CH2—CH2—NH2 or CH2—CH2—N(CH3)3, with a primary or secondary alcohol with a chain length of between C2 to C4, optionally substituted with one or more polar groups selected from the group consisting of amino, hydroxy and carboxy, in a single aqueous phase in the presence of an effective amount of phospholipase D with transphosphatidylation activity produced from a Streptomyces hachijoense strain to catalyze the reaction to obtain a phosphatide according to formula (I).
2 more elements
Dependencies are inferred from the claim text.