Isolated P27 protein, nucleic acid molecules encoding same, methods of identifying agents acting on same, and uses of said agents
AssigneesFred Hutchinson Institute for Cancer Research, Sloan-Kettering Institute for Cancer Research
InventorsJoan Massague, James M. Roberts, Andrew Koff, Kornelia Polyak
Life of this patent
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Abstract
An isolated protein designated p27 is disclosed. The p27 protein has an apparent molecular weight of about 27 kD, and is capable of binding to and inhibiting the activation of a cyclin E-Cdk2 complex. A nucleic acid sequence encoding p27 protein is disclosed, as well as a method for producing p27 in cultured cells. in vitro assays for discovering agents which effect the activity of p27 are also provided. Methods of diagnosing and treating hypoproliferative and hyperproliferative disorders are provided.
Claims19
An isolated nucleic acid comprising a nucleotide sequence represented in SEQ ID No. 1.
An isolated nucleic acid comprising a coding sequence for a polypeptide, which polypeptide includes an amino acid sequence represented in SEQ ID No. 2.
An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide including an amino acid portion at least 90% identical to residues +28 to +88 of SEQ ID No. 2, wherein said amino acid portion inhibits activation of a mammalian cyclin E-Cdk2 complex.
A nucleic acid comprising:
- (i) a nucleotide sequence encoding a polypeptide that inhibits activation of a mammalian cyclin E-Cdk2 complex, which polypepuide includes a portion at least 90% identical to residues +28 to +88 of SEQ ID No. 2;
- and (ii) a heterologous transcriptional regulatory sequence operably linked to the coding sequence.
A nucleic acid encoding a polypeptide having an amino acid sequence consisting essentially of residues 28-79 of SEQ ID No. 2, 4 or 6.
Dependencies are inferred from the claim text.