Production of recombinant lactoferrin and lactoferrin polypeptides using cDNA sequences in various organisms

A method for producing biologically active recombinant lactoferrin comprising the steps of:

• combining sequences containing a selectable marker gene, a promotor, a transcription termination sequence, and a linker sequence;
• cloning said sequences to form a plasmid;
• digesting said plasmid with a restriction endonuclease;
• inserting a cDNA coding for human, bovine or porcine lactoferrin into a restriction site;
• and
• transforming a cell with said plasmid to produce said recombinant lactoferrin.

A method for producing biologically active recombinant lactoferrin comprising the steps of:

• combining sequences containing a selectable marker gene, a promotor, a transcription termination sequence, and a linker sequence;
• cloning said sequences to form a plasmid;
• digesting said plasmid with a restriction endocnuclease;
• inserting a substitution analog of a cDNA sequence selected from the group consisting of SEQ. ID No. 1 into a restriction site;
• and transforming eucaryotic cells with said plasmid expressing lactoferrin cDNA which produces said recombinant lactofemin.