Hydrogen peroxide indicator employing enzyme and dye
AssigneeEthicon, Inc.
InventorsTodd Morrison, Veronica V. Thralls, Franklin A. Jurik
Life of this patent
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Abstract
A method for determining exposure of a test sample to hydrogen peroxide in an atmosphere includes placing a substrate into the atmosphere, contacting the substrate with the hydrogen peroxide, reacting the hydrogen peroxide with a dye intermediate to produce a chromophore indicative of the integrated exposure of hydrogen peroxide in the atmosphere; and correlating the chromophore to the integrated exposure of hydrogen peroxide in the atmosphere. The dye intermediate may be a dye-couple of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and 3-dimethylaminobenzoic acid (DMAB). The intensity of the chromophore is indicative of an integrated hydrogen peroxide exposure.
Claims16
A method for detecting the integrated exposure of an item to hydrogen peroxide in a sterilization chamber having an atmosphere, the method comprising the steps of:
- placing the item into the chamber;
- placing a substrate capable of absorbing hydrogen peroxide in the chamber;
- contacting the item and the substrate with the hydrogen peroxide;
- hydrating the substrate in the presence of a thickening agent;
- on the substrate, reacting the hydrogen peroxide with a dye intermediate and an enzyme capable of reacting with hydrogen peroxide to produce a chromophore indicative of the integrated exposure of hydrogen peroxide in the atmosphere;
- and
- correlating the chromophore to the integrated exposure of hydrogen peroxide in the atmosphere.
Dependencies are inferred from the claim text.