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US 6,635,435 B1Granted
Fluorogenic substrates and their use
AssigneeChromagen, Inc.
InventorsMichael J. Conrad, Liyan He
Life of this patent
Estimating the term…
Abstract
The subject invention provides compounds useful as fluorogenic substrates. Upon hydrolysis of a hydrolyzable group, a halo-pyrene substituted molecule is developed which is highly fluorescent, water soluble and exhibits several desirable characteristics, including a large Stokers' shift.
Claims9
A fluorogenic substrate molecule having the following characteristics:
- (a) said molecule re-emits at least about 70% of the energy of the incident radiation that is used to excite the molecule;
- (b) the molecule emits radiation at a wavelength that is at least about 25 nm from the wavelength of radiation used to excite the molecule;
- and
- (c) the molecule is soluble and stable in water;
- and wherein said molecule has the following structure:
- and salts thereof, wherein:
- R1 is selected from the group consisting of phosphate, sulfonamide, —O-galactoside, —O-glucoside, —O-glucuronide, and N-acetylglucosaminide;
- and
- R2, R3, and R4, can be the same or different, and are selected from the group consisting of SO3 and halides;
- provided that at least one of R2, R3, or R4 is a halide.
7 more elements
A method of detecting a molecule of interest, wherein said molecule is labeled with a fluorogenic substrate molecule which has the following characteristics:
- (a) said molecule re-emits at least about 70% of the energy of the incident radiation that is used to excite the molecule;
- (b) the molecule emits radiation at a wavelength that is at least about 25 nm from the wavelength of radiation used to excite the molecule;
- and
- (c) the molecule is soluble and stable in water;
- and wherein said molecule has the following structure:
- and salts thereof, wherein:
- R1 is selected from the group consisting of phosphate sulfonamide, and substrates for hydrolytic enzymes;
- and
- R2, R3, and R4 are, independently, selected from the group consisting of SO3 and halides;
- provided that at least one of R2, R3, or R4 is a halide;
- and wherein said method comprises labeling a molecule of interest with said fluorogenic substrate molecule, adding an enzyme to hydrolyze said fluorogenic substrate, and detecting a change in fluorescence.
8 more elements
Dependencies are inferred from the claim text.