Oligonucleotides and methods for detecting hepatitis B viral nucleic acids
AssigneeQuest Diagnostics Investments Incorporated
InventorsVijaya Pasupuleti, Hasnah Hamdan, Michael Lewinski
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Abstract
The present invention provides methods and compositions for determining the presence and/or amount of HBV nucleic acids in a test sample. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting HBV nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the HBV assay.
Claims6
A method for detecting the presence or amount of HBV nucleic acids in a test sample, comprising:
- (a) amplifying HBV nucleic acids if present in said sample using a pair of oligonucleotide primers having the sequences set forth in SEQ ID NO:1 and SEQ ID NO:2;
- (b) hybridizing said amplified HBV nucleic acids with an oligonucleotide probe having the sequence set forth in SEQ ID NO:7 in the presence of an enzyme that cleaves said probe when said probe hybridizes to said HBV nucleic acids;
- and
- (c) detecting a signal from said probe, wherein said signal indicates the presence or amount of HBV nucleic acids in said test sample.
Dependencies are inferred from the claim text.