Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
AssigneeStratagene
InventorsMichael H. Wigler, Joseph A. Sorge
Life of this patent
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Abstract
A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
Claims18
A diverse library of DNAs encoding families of antigen-combining proteins produced by the method of:
- (a) obtaining DNA containing genes encoding antigen-combining proteins;
- (b) combining the DNA containing genes encoding antigen-combining proteins with sequence specific primers which are oligonucleotides homologous to conserved regions of the genes;
- (c) performing sequence specific gene amplification that results in amplified genes;
- and
- (d) introducing the amplified genes into a framework antibody vector.
A diverse library of DNAs encoding families of antibodies produced by a method comprising:
- (a) obtaining DNA comprising genes encoding antibodies;
- (b) combining said DNA with sequence specific primers which are oligonucleotides homologous to conserved regions of the genes;
- (c) performing sequence specific gene amplification that results in amplified genes;
- and
- (d) introducing the amplified genes into a framework antibody vector.
Dependencies are inferred from the claim text.