Cryopreservation system with controlled dendritic freezing front velocity
AssigneeIntegrated Biosystems, Inc.
InventorRichard Wisniewski
Life of this patent
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Abstract
A biopharmaceutical material cryopreservation system is provided that includes a flexible sterile container comprising a biocompatible polymeric material. The flexible sterile container contains biopharmaceuticals materials for freezing and cryopreservation. A freezing system is thermally coupled to the biopharmaceutical materials via the flexible sterile container. The freezing system comprises a temperature sensor that monitors a temperature of the biopharmaceutical materials. Also, the freezing system comprises a feedback loop constructed to control a dendritic freezing front velocity, within the biopharmaceutical materials, in a range from approximately 5 millimeters per hour to approximately 250 millimeters per hour based on feedback information from the temperature sensor.
Claims34
A cryopreservation system, comprising:
- a biocompatible flexible container adapted to receive at least one biopharmaceutical material therein, and;
- a freezing system thermally coupled to the at least one biopharmaceutical material and/or the flexible container, said freezing system comprising a temperature sensor that monitors a temperature of the at least one biopharmaceutical material in the container,
- wherein the freezing system comprises a feedback loop constructed to control a dendritic freezing front velocity, within the at least one biopharmceutical material, in a range from approximately 5 millimeters per hour to approximately 250 millimeters per hour based on feedback information comprising the temperature detected by the temperature sensor.
A method for cryopreservation of biopharmaceutical materials by freezing the materials, comprising:
- providing a flexible container comprising a biocompatible material;
- receiving at least one biopharmaceutical material in said container;
- thermally coupling a freezing system to the at least one biopharmaceutical material and/or the flexible container, said freezing system comprising a temperature sensor that monitors a temperature of the at least one biopharmaceutical material in the container and a feedback loop constructed to control freezing;
- and
- freezing said at least one biopharmaceutical material and controlling dendritic freezing front velocity during said freezing, within the at least one biopharmceutical material, in a range from approximately 5 millimeters per hour to approximately 250 millimeters per hour based on feedback information comprising the temperature detected by the temperature sensor.
A cryopreservation system, comprising:
- flexible container means for receiving at least one biopharmaceutical material, said flexible container means comprising a biocompatible material, and;
- freezing means for freezing the at least one biopharmaceutical material, said freezing means thermally coupled to the at least one biopharmaceutical material via the flexible container, said freezing means comprising a temperature sensor that monitors a temperature of the at least one biopharmaceutical material in the container,
- wherein the freezing means comprises a feedback loop constructed to control a dendritic freezing front velocity, within the at least one biopharmaceutical material, in a range from approximately 5 millimeters per hour to approximately 250 millimeters per hour based on feedback information comprising the temperature detected by the temperature sensor.
A cryopreservation system, comprising:
- a biocompatible flexible container adapted to receive at least one biopharmaceutical material therein;
- a freezing system thermally coupled to the at least one biopharmaceutical material and/or the flexible container, said freezing system comprising a cryocooling enclosure having an interior configured to receive and support said biocompatible flexible container, said cryocooler adapted to receive a cryocoolant therein to cool the interior of said crycooling enclosure;
- one or more temperature sensors positioned to monitor a temperature relating to the at least one biopharmaceutical material in the container;
- and
- a feedback loop constructed to control a dendritic freezing front velocity, within the at least one biopharmaceutical material, in a range from approximately 5 millimeters per hour to approximately 250 millimeters per hour based on feedback information comprising the temperature detected by the one or more temperature sensors.
Dependencies are inferred from the claim text.