Transgenic animals for studying regulation of genes
AssigneeNovartis AG
InventorsEdgar Käslin, Marcel Luyten, Hans-Günter Zerwes
Life of this patent
Estimating the term…
Abstract
A transgenic non-human animal (e.g., a transgenic mouse) comprising a polynucleotide encoding a soluble marker protein functionally linked to a regulatory sequence of an endogenous gene encoding E-selectin. Also described are methods for detecting modulators of the E-selectin gene using said transgenic non-human animal, and cultured cells obtained therefrom.
Claims1
A transgenic mouse comprising a polynucleotide encoding a soluble marker protein functionally linked to a regulatory sequence of an endogenous gene encoding E-selectin, wherein the soluble marker protein is a secreted alkaline phosphatase, and wherein the polynucleotide encoding the secreted alkaline phosphatase is inserted into a region of an E-selectin gene of a chromosomal E-selectin allele of said mouse, which is between a transcription start site and a translation start site of said E-selectin gene, and wherein said mouse expresses the secreted alkaline phosphatase by endothelial cells and said expression is regulatable by chemical stimulus or physical stimulus.
Dependencies are inferred from the claim text.