Method for the production of in vivo model of hepatocellular carcinoma without lung metastasis
AssigneeKabushiki Kaisha Daiyu-Kai Institute of Medical Science
InventorsTomoyuki Shirai, Mitsuru Futakuchi
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Abstract
The present invention provides a method for producing an in vivo model of hepatocellular carcinoma without lung metastasis in an animal by injecting diethylnitrosamine and administering nitrosomorpholine in the drinking water of the animal, as well as, methods of identifying an antimetstatic agent using the in vivo model.
Claims19
A method of producing an in vivo model of hepatocellular carcinoma without lung metastasis in a rat, comprising injecting from 50 to 200 mg/kg of diethylnitrosoamine into the intraperitonium of the rat;
- followed by administering nitrosomorpholine to the rat by mixing 40 to 120 ppm of the nitrosomorpholine into drinking water consumed by the rat for a time sufficient to produce hepatocellular carcinoma without lung metastasis in the rat.
A method of identifying an antimetastic agent comprising,
- producing an in vivo model of hepatocellular carcinoma without lung metastasis in a rat, comprising
- (a) injecting from 50 to 200 mg/kg of diethylnitrosoamine into the intraperitonium of the rat;
- (b) followed by administering nitrosomorpholine to the rat by mixing 40 to 120 ppm of the nitrosomorpholine into drinking water consumed by the rat for a time sufficient to produce hepatocellular carcinoma without lung metastasis in the rat;
- (c) administering an agent to the rat;
- and
- (d) detecting the onset of lung metastasis in the rat, wherein a delayed or reduced onset of lung metastasis in the rat compared to a rat model of hepatocellular carcinoma without lung metastasis produced as in step (a) and (b) in which the agent in step (c) has not been administered is indicative of an antimetastatic effect by the agent.
Dependencies are inferred from the claim text.