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AssigneesProdiGene, Inc., Genencor International, Inc.
InventorsElizabeth Hood, John A. Howard, Michele Bailey, Franciscus J. C. van Gastel, Michael Ward, Huaming Wang, Susan Woodard
Estimating the term…
A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.
A method of improving recovery of active enzyme from a corn plant where the enzyme requires a transitional metal cofactor for activity comprising introducing into the plant nucleotide sequences encoding the enzyme, the enzyme selected from the group consisting of laccase or organophosphate hydrolase, comprising extracting the enzymes from the plant and exposing or treating the enzyme with the metal cofactor.
A method of improving the levels of active enzyme in a plant where the enzyme requires a transitional metal cofactor for activity, comprising introducing into the corn plant a nucleotide sequence encoding the enzyme, the enzyme selected from the group consisting of laccase or organophosphate hydrolase comprising exposing the plant to a solution containing the metal cofactor during plant development.
A method of improving isolated of active laccase from a corn plant comprising introducing into the plant a nucleotide sequence encoding laccase and exposing the laccase to copper.
A method of improving recovery of active organophosphate hydrolase from a plant comprising introducing into the corn plant nucleotide sequences encoding organophosphate hydrolase and exposing the organophosphate hydrolase to a metal cofactor.
Dependencies are inferred from the claim text.