Avidin derivatives and uses thereof
AssigneeYeda Research and Development Co. Ltd.
InventorsMeir Wilchek, Edward A Bayer, Heike Hofstetter, Margherita Morpurgo
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Abstract
A covalent conjugate of a 4′-hydroxyazobenzene-2-carboxylic acid derivative (HABA) and an avidin-type molecule, of the formula: wherein A is (CH2)n or —CH═CH—, wherein n is an integer from 0-10; B is (CH2)n wherein n is an integer from 2 to 10; m is zero or 1; and Av is the residue of an avidin-type molecule selected from the group comprising native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, truncated streptavidin and other derivatives of said avidin-type molecules. These HABAylated avidins are red colored in the quinone configuration and can be used in many applications in the avidin-biotin technology.
Claims15
A covalent conjugate of a 4′-hydroxyazobenzene-2-carboxylic acid derivative (hereinafter HABA) and an avidin-type molecule, said conjugate being referred herein as “HABAylated avidin” and having the formula:
- wherein
- A is (CH2)n or —CH═CH—, wherein n is an integer from 0-10;
- B is (CH2)n wherein n is an integer from 2 to 10;
- m is zero or 1;
- and
- Av is the residue of an avidin-type molecule selected from the group consisting of native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, and truncated streptavidin.
A cyclic HABA derivative of the formula III:
- wherein A is (CH2)n or —CH═CH—, wherein n is an integer from 0-10;
- and B is (CH2)n wherein n is an integer from 2 to 10.
Dependencies are inferred from the claim text.