One-step immunoassay for the determination of antigen-specific antibodies of one of the immunoglobulin classes A, M, D, or E, and an agent suitable for this purpose

Abstract

An immunochemical method for the determination of antibodies which are specific for an antigen and are of one of the immunoglobulin classes: A, M, D or E in a fluid, with this fluid being contacted with a solid phase to which the antibodies against this immunoglobulin class, or a fragment of an antibody of this type, are bound, which results in the immunoglobulin of this class being bound to this solid phase, and this solid phase being contacted with the antigen, which carries a labeling means where appropriate, and with a labeled antibody or a labeled fragment of an antibody against the antigen if the antigen is unlabeled and determination, from the amount of labeling means which is bound to the solid phase, of the amount of these antibodies which are specific for an antigen and are one of the immunoglobulin classes, which comprises the solid phase being simultaneously in contact with the fluid containing the antibody which is to be determined and with the antigen, which is labeled where appropriate, there being addition of a substance which prevents immunoglobulin G binding to the solid phase and, where appropriate, antigen binding to immunoglobulin G, and an agent suitable for this purpose, are described.

Dependencies are inferred from the claim text.