Nanoparticle encapsulation system and method

A method of forming a dispersion of surfactant micelles, the method comprising:

• dispersing surfactant molecules into a first hydrophilic composition, the first hydrophilic composition comprising a hydrophilic bioactive component, wherein the surfactant molecules have an HLB value of less than about 6.0 units, and wherein the surfactant molecules form a shell around the hydrophilic bioactive component to form a dispersion of surfactant micelles;
• adding a biocompatible hydrophilic polymer to the dispersion of surfactant micelles;
• and
• wherein the biocompatible hydrophilic polymer stabilizes the dispersion.

A method of forming a particle, the method comprising:

• dispersing a surfactant molecule into an aqueous composition comprising a hydrophilic bioactive component, wherein the surfactant molecule has an HLB value of less than about 6.0 units, and wherein the surfactant molecule is associated with the hydrophilic bioactive component to form a plurality of surfactant micelles;
• exposing the surfactant micelles to a biocompatible polymer to form a plurality of stabilized surfactant micelles;
• and
• solidifying the stabilized surfactant micelles to form particles by exposing the stabilized surfactant micelles to at least one cation, wherein the particles have an average diameter of less than about 50 nanometers as measured using atomic force microscopy of the particles following drying of the particles.

A method of making a particle, the method comprising:

• condensing a bioactive component to form a condensed bioactive component;
• dispersing a surfactant into an aqueous composition comprising the condensed bioactive component, wherein the surfactant has an HLB value of less than about 6.0 units, and wherein the surfactant associates with the condensed bioactive component to form a plurality of surfactant micelles;
• exposing the surfactant micelles to a biocompatible polymer to form a plurality of stabilized surfactant micelles;
• and
• precipitating the stabilized surfactant micelles to form particles having an average diameter of less than about 50 nanometers as measured by atomic force microscopy of the particles following drying of the particles;
• wherein the bioactive component comprises DNA or a polypeptide.

A method of preparing particles, the method comprising:

• forming a plurality of hydrophobic compositions, wherein the hydrophobic composition comprises a surfactant associated with a bioactive hydrophobic component, and wherein the surfactant has an HLB value of less than about 5.0 units;
• adding a biocompatible polymer to the hydrophobic composition to form a stabilized composition;
• and
• precipitating the stabilized composition to form a particle having an average diameter of less than about 50 nanometers as measured using atomic force microscopy of dried particles.

A method of forming a particle, the method comprising:

• dispersing a surfactant molecule into an aqueous composition comprising a hydrophilic bioactive component, wherein the surfactant molecule has an HLB value of less than about 5.0 units, and exposing the surfactant molecule to the hydrophilic component to form a plurality of surfactant micelles;
• adding a biocompatible polymer to the plurality of surfactant micelles to form a plurality of stabilized surfactant micelles;
• solidifying the stabilized surfactant micelles to form a plurality of particles by exposing the stabilized surfactant micelles to at least one cation, wherein the particles have an average diameter of less than about 50 nanometers as measured by atomic force microscopy of the particles following drying of the particles;
• and
• applying a cell recognition component to the particles.

A method of forming a targeted particle, the method comprising:

• dispersing a surfactant molecule into an aqueous composition comprising a bioactive molecule, wherein the surfactant molecule has an HLB value of less than about 6.0 units, and wherein the surfactant molecule associates with the bioactive molecule to form a surfactant micelle;
• adding a biocompatible polymer and a cell recognition component to the surfactant micelle to form a stabilized aqueous composition comprising a plurality of stabilized surfactant micelles;
• and
• exposing the stabilized surfactant micelles to at least one cation to precipitate the stabilized surfactant micelles to form particles having an average diameter of less than about 50 nanometers as measured by atomic force microscopy of the particles following drying of the particles.

A method of forming a particle, the method comprising:

• dispersing a surfactant molecule into an aqueous composition comprising a hydrophilic biocompatible component, wherein the surfactant molecule has an HLB value of less than about 6.0 units, and wherein the surfactant molecule is associated with the hydrophilic biocompatible component to form a plurality of surfactant micelles;
• exposing the surfactant micelles to a biocompatible polymer to form a plurality of stabilized surfactant micelles;
• and
• solidifying the stabilized surfactant micelles to form particles by exposing the stabilized surfactant micelles to at least one cation, wherein the particles have an average diameter of less than about 50 nanometers as measured using atomic force microscopy of the particles following drying of the particles.