Monolayer sheet structure of primary hepatocytes obtained using a protein-phosphorylation inhibitor
AssigneeJapan as represented by Secretary of Agency of Industrial Science and Technology
InventorsSumio Maeda, Hidetoshi Inagaki, Takao Saito
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Abstract
A monolayer sheet structure is prepared containing a confluent monolayer of primary hepatocytes cultured on a substrate in sheet form. The monolayer sheet structure is formed by treating the hepatocytes in the monolayer formation stage with a protein-phosphorylation inhibitor having an indolo [2,3-a] carbazole structure, such as staurosporine, or other similar structure.
Claims18
A monolayer sheet structure comprising a confluent monolayer comprising primary hepatocytes cultured on a substrate in sheet form, wherein said monolayer sheet structure of primary hepatocytes is produced by treating the hepatocytes with a protein-phosphorylation inhibitor having an indolo [2,3-a] carbazole skeleton during formation of the monolayer.
A monolayer sheet structure consisting of a confluent monolayer comprising primary hepatocytes cultured on a substrate in sheet form, wherein
- said monolayer sheet structure of primary hepatocytes is produced by treating the hepatocytes with a protein-phosphorylation inhibitor having an indolo [2,3-a] carbazole skeleton during formation of the monolayer.
A method of manufacturing a monolayer sheet structure comprising culturing primary hepatocytes on a culture substrate in sheet form to produce a confluent monolayer, and
- treating the hepatocytes with a protein-phosphorylation inhibitor having an indolo [2,3-a] carbazole skeleton during formation of the monolayer to form the monolayer sheet structure of primary hepatocytes.
The method according to clam 6, further comprising isolating primary hepatocytes from livers or liver sections by a collagenase perfusion method.
Dependencies are inferred from the claim text.