Normothermic, hypothermic and cryopreservation maintenance and storage of cells, tissues and organs in gel-based media
AssigneeBioLife Solutions, Inc.
InventorsJohn M. Baust, Robert Van Buskirk, John G. Baust
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Abstract
Gel-based medium compositions and a method of use thereof in normothermic, hypothermic or cryopreservative storage and transport of cell samples are described. These gel-based compositions contain a cell maintenance and preservation medium together with a gelling agent. Such gel-based medium compositions protect various cell samples, such as animal or plant organs, tissues and cells, from the mechanical, physiological and biochemical stresses inherently associated with liquid preservation techniques.
Claims6
A method for increasing a storage duration of a cell sample at frozen temperatures, the method comprising:
- forming a cell pellet;
- mixing the pellet with a cell preservation medium comprising a cryopreservant, said medium not containing a gelling agent;
- adding a medium containing a gelling agent to the mixture of cells and cell preservation medium, the medium containing the gelling agent being in a liquid state when added;
- reducing the temperature of the resulting mixture until the mixture gels;
- and
- storing the gelled mixture at a temperature below 0° C.
Dependencies are inferred from the claim text.