Modified subtilisins and detergent compositions containing same
AssigneesNovozymes A/S, Unilever PLC
InventorsDorrit Aaslyng, Sven Branner, Sven Hastrup, Leif Nørskov-Lauritsen, Ole Hvilsted Olsen, Merete Simonsen, Eric Casteleijn, Maarten Robert Egmond, Johan Haverkamp, John David Marugg, Arnoldus Theodorus Anthonius Mooren
Life of this patent
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Abstract
Enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.
Claims87
A modified subtilisin comprising a substitution of an amino acid with a different amino acid at one or more positions selected from the group consisting of:
- 27, 105, 106, 141, 251, 256, and 257,
- wherein each position corresponds to the position of the amino acid sequence of the mature subtilisin BPN′.
A modified subtilisin comprising an insertion of an amino acid at position 36, wherein position 36 corresponds to the position of the amino acid sequence of the mature subtilisin BPN′.
A modified subtilisin comprising a mutation in an amino acid sequence of a subtilisin at a position numbered according to the amino acid sequence of the mature subtilisin BPN′, wherein the mutation is selected from the group consisting of:
- Q12K, Q12R, R19Q, T22R, D32*, T38K, T38R, R45A, E53G+K235L, E53K, E53R, E54G, E54T, E54Y, Q59E, T71D, T71D+G195E, E89S, G97D, G97D+H120K, N97D, N97D+S98D, N97D+T213D, A98K, A98R, S98D, S98D+T213D, S99D, S99D+N140K, D120K, H120D+G195E, H120D+G195E+K235L+K251E, H120D+R170Y+G195E+K235L+K251E, H120K, P129D, D140K, N140K, N140R, S156E, A158K, A158R, Y167V, R170Y+K251E, Y171T, D172K, R186P G195E+T213R, G195F, D197E, D197K, D197N, Q206D, Q206E, T213D, Y214S, Y214T, K235L, K235R, K237R, W241L, and S265R.
Dependencies are inferred from the claim text.