Thermophilic DNA polymerases from Thermoactinomyces vulgaris
AssigneePromega Corporation
InventorsTrent Gu, Fen Huang, James Robert Hartnett
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Abstract
The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
Claims13
A composition comprising a purified and isolated DNA polymerase;
- wherein said DNA polym erase has the amino acid sequence set forth in SEQ ID NO:2;
- and wherein said DNA polymerase has DNA synthesis activity.
A purified thermostable mutant DNA polymerase comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 4 and SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.