Use of α-1,4-glucan lyase for preparation of 1,5-D-hydrofructose
AssigneeDanisco A/S
InventorsShukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen, Tove Christensen
Life of this patent
Estimating the term…
Abstract
A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an α-1,4-glucan with an α-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the α-1,4-links, the α-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.
Claims24
A method of preparing 1,5-D-anhydrofructose comprising treating an α-1,4-glucan with a purified α-1,4-glucan lyase, said α-1,4-glucan lyase comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:5.
Dependencies are inferred from the claim text.