Vectors and methods for recombinant protein expression
AssigneeImmunex Corporation
InventorJeffrey T. McGrew
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Abstract
There is disclosed an expression vector utilizing an internal polyadenylation signal. The internal polyadenylation signal is inserted between a DNA encoding a protein of interest and a DNA encoding a selectable marker, and allows a single promoter to generate both monocistronic messages and dicistronic messages. Similar, multicistronic vectors can also be prepared. Also disclosed are methods of using the expression vector utilizing an internal polyadenylation signal, host cells transfected therewith, stable pools of cells transfected with an expression vector utilizing an internal polyadenylation signal, and clones of such transfected cells.
Claims44
An expression vector comprising, in the following order, a promoter sequence, a first coding sequence, a polyadenylation site, and a second coding sequence operably linked to the first coding sequence, wherein no promoter sequence occurs between the polyadenylation site and the second coding sequence.
An expression vector comprising, in the following order, a promoter sequence, a first coding sequence, a polyadenylation site comprising nucleotides 123 through 128 and 151 through 187 of SEQ ID NO:1, and a second coding sequence, wherein no promoter sequence occurs between the polyadenylation site and the second coding sequence.
Dependencies are inferred from the claim text.