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US 6,632,635 B1Granted
Methods of refolding proteins
AssigneeGenetics Institute, Inc.
InventorSteven M. Vicik
Life of this patent
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Abstract
Methods are disclosed for refolding proteins of the TGF-β family of proteins. The methods employ as refolding agents one or more compounds which are acid substituted aminocyclohexanes.
Claims5
A method of expressing a protein from the transforming growth factor-beta (TGF-β) superfamily comprising:
- a) culturing prokaryotic cells which have been transformed with a DNA encoding a TGF-β protein under conditions suitable for the production of recombinant TGF-β protein;
- and
- b) refolding the recombinantly produced TGF-β protein in refolding media comprising 2-aminocyclohexanecarboxylic acid.
Dependencies are inferred from the claim text.