Decay accelerating factor (DAF) and nucleic acid encoding it
AssigneeGenentech, Inc.
InventorsIngrid W. Caras, Michael A. Davitz, Victor Nussenzweig, David W. Martin, Jr.
Life of this patent
Estimating the term…
Abstract
Novel fusions of a GPI signal domain and a polypeptide heterologous to the GPI signal domain donor polypeptide are provided for industrial use. Therapeutic administration of the GPI-linked product of the fusions enables the targeting of biological activity to cell membrane surfaces.
Claims22
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a decay accelerating factor (DAF) having an amino acid sequence shown in FIGS. 1A-D (SEQ ID NO:2) or FIGS. 2A-E (SEQ ID NO:4) or an antigenic fragment thereof.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a DAF variant comprising one or more amino acid deletions from the amino acid sequence shown in FIGS. 1A-D (SEQ ID NO:2) or FIGS. 2A-E (SEQ ID NO:4) or an antigenic fragment thereof, wherein said amino acid deletions are selected from the group of deletions consisting of des-Cys330, des-Cys330-Thr347, and des-Thr2-Gly327.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a DAF variant comprising one or more amino acid. substitutions within the amino acid sequence shown in FIGS. 1A-D (SEQ ID NO:2) or FIGS. 2A-E (SEQ ID NO:4) or an antigenic fragment thereof, wherein said amino acid substitutions are selected from the group consisting of Cys330 4 Met, Cys330 4 Ser, Cys2→Ser, Lys125Lys126→Gln, Gly144→Pro, lle146→Met, Phe169→Tyr, Pro192→Gly, Ile201→Leu, Asn236Asn237→AspAsp, Glu239→Asp, Ser256→Tyr, Val268→Phe, Lys285→Gln, Thr294→Ser, and Leu324→Ser.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a sDAF variant comprising one or more amino acid substitutions within the amino acid sequence shown in FIGS. 2A-E (SEQ ID NO:
- 4) or an antigenic fragment thereof, wherein said amino acid substitutions are selected from the group consisting of Lys352→Gln, Cys339→Ser, Arg394→His, and Leu403Phe404Leu405→SerTyrSer.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a DAF variant comprising one or more amino acid insertions in the amino acid sequence shown in FIGS. 1A-D (SEQ ID NO:2) or FIGS. 2A-E (SEQ ID NO:4) or an antigenic fragment thereof, wherein said amino acid insertions are selected from the group consisting of LeuLeu inserted between Thr329 and Cys330;
- His inserted between Arg100 and Arg101;
- Gln inserted between Lys125 and Lys126 and Gln inserted between Lys126 and Lys127;
- LeuLeu inserted between Pro193 and Ala,194;
- AspAsp inserted between Pro247 and Glu248;
- SerSer inserted between Thr282 and Thr283;
- ThrThr inserted between Gly316 and Thr317;
- and N-terminal methionyl.
Dependencies are inferred from the claim text.