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US 6,632,625 B1Granted
Mutant OB receptors and nucleotides encoding them
AssigneeMerck & Co., Inc.
InventorsTung M. Fong, Ruey-Ruey C. Huang, Leonardus Van Der Ploeg
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Abstract
Mutant ob receptors have been made which a) lack a functional first CK-F3 domain; b) lack a functional second CK-F3 domain or c) lack a functional intracellular domain. These receptors may be used in various assays, such as a transactivation assay to identify novel ligands.
Claims11
A mutant ob receptor which differs from a wild-type receptor by reason of a deletion of amino acid residues which comprise all or substantially all of the first CK-F3 module.
A method for determining if an OB-R ligand is present in a sample comprising:
- a) contacting a cell with the sample wherein the cell expresses a mutant OB-R lacking all or substantially all of the first CK-F3 module;
- and wherein the cell also comprises a reporter gene construct comprising a leptin response element operatively linked to a reporter gene;
- and
- b) determining if transcription or translation of the reporter gene occurs wherein transcription or translation indicates an OB-R ligand is present in the sample.
1 more element
Dependencies are inferred from the claim text.