G protein-coupled receptor-like receptors and modulators thereof
AssigneePharmacia & Upjohn Company
InventorsDavid E. Lowery, Timothy G. Geary, Teresa M. Kubiak, Martha J. Larsen
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Abstract
The invention provides neuropeptide ligands, G protein-coupled receptors and methods of screening for modulators of receptor activity. Identified modulators, including neuropeptide ligand mimetics, are useful as biostatic and biocidal agents of varying scope, ranging from lethal activity restricted to particular invertebrate parasites to broad spectrum invertebrate parasiticides active on a wide range of invertebrates, including helminths and insects.
Claims21
A method of identifying a modulator of an activity of a GPCR-like receptor comprising the following steps:
- (a) contacting a test compound with a composition, wherein said composition comprises an invertebrate GPCR-like receptor polypeptide encoded by a polynucleotide having a sequence that is at least 98% identical to the sequence of SEQ ID NO:21;
- and
- (b) measuring the activity of said GPCR-like receptor in the presence and absence of said test compound wherein if the test compound alters a GPCE-like receptor activity, the test compound is identified as a modulator.
A method of identifying a candidate anti-invertebrate modulator comprising the steps of:
- (a) contacting a test compound with a composition, wherein said composition comprises an invertebrate GPCR-like receptor polypeptide encoded by a polynucleotide having a sequence that is at least 98% identical to the sequence of SEQ ID NO:21;
- and
- (b) identifying a test compound that binds to or interacts with said composition as a candidate anti-invertebrate modulator.
An isolated GPCR-like receptor consisting of the amino acid sequences of SEQ ID NO:22.
An isolated polynucleotide encoding a GPCR-like receptor wherein the polynucleotide comprises a sequence that is at least 98% identical to SEQ ID NO:21.
Dependencies are inferred from the claim text.