US 6,632,618 B1 · Morphogenic protein-specific cell surface receptors and uses therefor

Abstract

Disclosed are (1) nucleic acid sequences, amino acid sequences, homologies, structural features and various other data characterizing a morphogen cell surface receptors particularly OP-1-binding cell surface receptors ALK-2, ALK-3, and ALK-6; (2) methods for producing receptor proteins, including fragments thereof, using recombinant DNA technology; (3) methods for identifying novel morphogen receptors and their encoding DNAS; (4) methods and compositions for identifying compounds capable of modulating endogenous morphogen receptor levels; and (5) methods and compositions for identifying morphogen receptor binding analogs useful in the design of morphogen agonists and antagonists for therapeutic, diagnostic and experimental uses.

Claims6

Dependent claims

A method for identifying an OP-1 analog having the same binding affinity for a cell surface receptor as OP-1, the method comprising the steps of:

(a) providing a sample comprising cells flat express a surface receptor having binding specificity foe OP-1, said receptor selected from the group consisting of:
(i) a polypeptide comprising amino acid residues 16-123 of SEQ ID NO:
4 (ALK-2);
(ii) a polypeptide comprising amino acid residues 24-152 of SEQ ID NO:
6 (ALK-3);
(iii) a polypeptide comprising amino acid residues 23-122 of SEQ ID NO:
8 (ALK-6);
and
(iv) a polypeptide having binding affinity for OP-1 and encoded by nucleic acids 256-552 of SEQ ID NO:
7 (ALK-6);
(b) contacting said sample with a candidate OP-1 analog;
and
(c) detecting binding affinity of said candidate to an OP-1 cell surface receptor;
wherein an OP-1 analog is identified as a candidate having the samed binding affinity for said receptor as OP-1.

11 more elements

Dependencies are inferred from the claim text.

Morphogenic protein-specific cell surface receptors and uses therefor

Life of this patent

Morphogenic protein-specific cell surface receptors and uses therefor

Life of this patent

Abstract

Claims6