Hybridization assays on oligonucleotide arrays
AssigneeAffymetrix, Inc.
InventorsMaureen T. Cronin, Garry Miyada, Mark Trulson, Thomas R. Gingeras, Glenn McGall, Claire Robinson, Michelle Smedsrud-Oval
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Abstract
This invention provides methods of performing nucleic acid hybridization assays on high-density substrate-bound oligonucleotide arrays involving including in the hybridization mixture an isostabilizing agent, a denaturing agent or a renaturation accelerant.
Claims22
A method of detecting known polymorphic differences in a target nucleic acid molecule by performing a hybridization assay between the target nucleic acid molecule and a probe array wherein the probe array comprises a surface to which are covalently attached probes with different, known nucleotide sequences, at discrete, known locations;
- the method comprises incubating the array with a hybridization mixture comprising the target nucleic acid molecule and betaine, determining the identity of the probes to which the target has hybridized and determining whether the target nucleic acid has a known polymorphic difference.
A method for performing a hybridization assay between a target nucleic acid molecule and a probe array, wherein the probe array comprises a surface to which are covalently attached probes with different, known nucleotide sequences, at discrete, known locations;
- the method comprises incubating the array with a hybridization mixture comprising the target nucleic acid molecule and betaine and determining the identity of probes to which the target has hybridized;
- wherein the target nucleic acid molecule is a naturally-occurring nucleic acid molecule.
Dependencies are inferred from the claim text.