Detection and determination of solid phase-associated factors

A method for the simultaneous detection of the presence or amount of multiple cell-associated factors, which are associated with the same cell, said method comprising:

• 1) providing a sample containing a cell comprising at least one cell-associated factor Fx and at least one cell-associated factor Fy;
• 2) selecting a receiver R and a transmitter T which enter into interaction with each other when in spatial proximity, and
• wherein the interaction directly or indirectly provides a signal;
• 3) providing at least one stable receiver complex, Lx-R, comprising the receiver R, and at least one ligand Lx,
• wherein Lx has binding affinity for the at least one cell-associated factor Fx;
• 4) providing at least one stable transmitter complex, Ly-T, comprising the transmitter T, and at least one ligand Ly,
• wherein Ly has binding affinity for the at least one cell-associated factor Fy;
• 5) contacting the sample with the at least one stable receiver complex and the at least one stable transmitter complex,
• wherein, in the presence of the at least one cell-associated factor Fx and Fy, complexes Fx-Lx-R and Fy-Ly-T are formed and;
• 6) detecting a signal provided by the interaction of T and R,
• wherein the detection of the signal correlates with the detection of the presence or amount of multiple cell-associated factors.