Recombinant poxvirus cytomegalovirus, compositions, and uses
AssigneeVirogenetics Corporation
InventorsEnzo Paoletti, Steven E. Pincus, William I. Cox, Elizabeth K. Kauffmann
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Abstract
Attenuated recombinant viruses containing DNA encoding an HCMV antigen, as well as methods and compositions employing the viruses, expression products therefrom, and antibodies generated from the viruses or expression products, are disclosed and claimed. The recombinant viruses can be NYVAC or ALVAC recombinant viruses. The recombinant viruses and gene products therefrom and antibodies generated by the viruses and gene products have several preventive, therapeutic and diagnostic uses. The DNA of the recombinant viruses can be used as probes or for generating PCR primers.
Claims11
An insertion plasmid comprising at least one DNA sequence coding for a HCMV protein, a promoter located upstream of the DNA sequence which is capable of inducing expression of the HCMV protein in a poxvirus, and flanking DNA sequences;
- wherein the poxvirus is selected from a group consisting of:
- (i) recombinant vaccinia virus wherein regions C7L-K1L, J2R, B13R+B14R, A26L, A56R and I4L have been deleted therefrom, or wherein the open reading frames for the thymidine kinase gene, the hemorrhagic regions, the A type inclusion body region, the hemegglutinin gene, the host range gene region, and the large subunit, ribonucleotide reductase have been deleted therefrom;
- (ii) NYVAC vaccinia virus;
- (iii) ALVAC canarypox virus;
- and
- (iv) attenuated canarypox virus wherein said canarypox virus is attenuated through more than 200 serial passages on chick embryo fibroblasts, a master seed therefrom was subjected to four successive plaque purifications under agar, from which a plaque clone was amplified through five additional passages.
An insertion plasmid selected from the group consisting of 542CMVgB, 553H6CMVgB, 553H6CMVgBTM−, 553H6gBC−TM−, I4L42 KgH, COPAKH6IE, COPAKH6IEN−, I4LH6IE-Ex4, I4LH6IEd32, pCMV65.2, plasmid 150.7, I4L42KgHH6IE-Ex4, and 542CMVgBgL.
An insertion plasmid selected from the group consisting of CP3LCMVgB, NVQC5L42KgH, NVQH6IE-Ex4, NVQH6IEd32, CMV65C6.2, plasmid 150.6, and plasmid 150.8.
Dependencies are inferred from the claim text.