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US 6,632,426 B2Granted
G-CSF analog compositions and methods
AssigneeAmgen Inc.
InventorTimothy David Osslund
Life of this patent
Estimating the term…
Abstract
The present invention relates to granulocyte colony-stimulating factor (“G-CSF”) hybrid molecules which retain the internal core helices of G-CSF. Also provided are pharmaceutical compositions containing hybrid molecules.
Claims4
A G-CSF molecule, optionally with an N-terminal methionyl residue, comprising:
- (a) a G-CSF internal core of helices A, B, C and D as set forth in FIG. 4, said internal core helices comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2, optionally with the cysteine residue at position 18 altered to serine or alanine residue;
- (b) an external loop between helices A and B comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (c) an external loop between helices B and C comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (d) an external loop between helices D and A;
- and
- (e) a N-terminus and a C-terminus, said N-terminus being connected to helix D and said C-terminus being connected to helix C, said N-terminus and said C-terminus each comprising a portion of amino acids 120-146 of SEQ. ID. NO. 2.
3 more elements
a G-CSF molecule, optionally with an N-terminal methionyl residue, comprising:
- (a) a G-CSF internal core of helices A, B, C and D as set forth in FIG. 4, said internal core helices comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2, optionally with the cysteine residue at position 18 altered to serine or alanine residue;
- (b) an external loop between helices C and D comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (c) an external loop between helices B and C comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (d) an external loop between helices D and A;
- and
- (e) a N-terminus and a C-terminus, said N-terminus being connected to helix B and said C-terminus being connected to helix A, said N-terminus and said C-terminus each comprising a portion of amino acids 39-73 of SEQ. ID. NO. 2.
3 more elements
A G-CSF molecule, optionally with an N-terminal methionyl residue, comprising:
- (a) a G-CSF internal core of helices A, B, C and D as set forth in FIG. 4, said internal core helices comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2, optionally with the cysteine residue at position 18 altered to seine or alanine residue;
- (b) an external loop between helices A and B comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (c) an external loop between helices C and D comprising corresponding amino acid residues as set forth in SEQ. ID. NO. 2;
- (d) an external loop between helices D and A;
- and (e) a N-terminus and a C-terminus, said N-terminus being connected to helix C and said C-terminus being connected to helix B, said N-terminus and said C-terminus each comprising a portion of amino acids 92-101 of SEQ. ID. NO. 2.
2 more elements
Dependencies are inferred from the claim text.