Diagnosis method of inflammatory, fibrotic or cancerous disease using biochemical markers

A method for diagnosis of liver fibrosis and/or presence of liver necroinflammatory lesions in a patient comprising the steps of:

• a) studying at least 4 biochemical markers by measuring the values of their concentration in the serum or plasma of said patient, wherein said markers are chosen from the group consisting of α2-macroglobulin, AST (aspartate aminotransferase), ALT (alanine aminotransferasc), GGT (gammaglutamyl transpeptidase), γ-globulin, total bilirubin, albumin, α1-globulin, α2-globulin, haptoglobin, β-globulin, apoA1, IL-10, TGF-β1, apoA2, and apoB;
• b) combining said values through a logistic function including said markers in order to obtain an end value, wherein said logistic function is obtained through the following method:
• i) classification of a cohort of patients in different groups according to the extent of their disease;
• ii) identification of factors which differ significantly between these groups by unidimensional analysis;
• iii) logistic regression analysis to assess the independent discriminative value of markers for the diagnosis of fibrosis and/or liver necroinflammatory lesions;
• iv) construction of the logistic function by combination of these identified independent factors;
• and
• c) analyzing said end value of said logistic function in order to determine the presence of liver fibrosis and/or liver necroinflammatory lesions in said patient.