Hepatitis C virus culture system
OwnerRalf Bartenschlagerinventor
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Abstract
The hepatitis C virus (HCV) cell culture system according to the invention consists of human hepatoma cells, which are transfected with a HCV-RNA construct, that comprises the HCV specific RNA segments 5′to NTR, NS3, NS4A, NS4B, NS5A, NS5B, and 3′to NTR as well as a minimum of one marker gene for selection (selection gene).
Claims17
A hepatitis-C-virus (HCV) RNA construct with the ability to replicate in eukaryotic cells, said HCV RNA construct is encoded by the nucleotide sequence depicted in SEQ ID NO:10, said nucleotide sequence coding for the HCV specific RNA-segments 5′to NTR, NS3, NS4A, NS4B, NS5A, NS5B and 3′to NTR and additionally for a marker gene for selection, wherein the marker gene for selection is the neomycin phosphotransferase gene.
A hepatitis-C-virus (HCV) RNA construct with the ability to replicate in eukaryotic cells, said HCV RNA construct comprising portions of the nucleotide sequence depicted in SEQ ID NO:10, said portions coding for the HCV specific RNA-segments 5′to NTR, NS3, NS4A, NS4B, NSSA, NS5S and 3′to NTR, and said HCV RNA construct further comprising a marker gene for selection or a reporter gene.
Dependencies are inferred from the claim text.