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US 6,630,314 B2Granted
Noninvasive detection of colorectal cancer and other gastrointestinal pathology
OwnerPadmanabhan P Nairinventor
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Abstract
A method for isolating viable, biologically substantially pure exfoliated fecal colonocytes at normal ambient temperature is described. Immunocoprocytes and inflammatory cells indicative of certain gastrointestinal conditions and a noninvasive method for detecting colorectal cancer are set forth. Composition of transport and suspension media for isolation of colonocytes are detailed.
Claims2
A method of screening for colorectal cancer, comprising the steps of:
- (a) collecting a fecal sample in a transport medium at normal ambient temperature;
- (b) dispersing the fecal sample in said transport medium diluted with a suspension medium;
- (c) sedimenting cells present in the diluted transport medium of step (b) to isolate the cells from impurities by layering the cell suspension over a medium of heavier density;
- (d) centrifuging the cells in step (C) to form a cellular band at a boundary with said heavier medium and within the heavier medium and pellet, thereby obtaining isolated intact viable exfoliated colonocytes from said fecal sample;
- then
- (e) reacting said isolated colonocytes with a reagent determinative of the presence of a marker of colorectal cancer, occurrence of a positive reaction of said colonocytes with said reagent being indicative of the presence of said cancer.
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Dependencies are inferred from the claim text.