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US 6,627,798 B2Granted
Aromatic amino acid biosynthetic enzymes
AssigneeE.I. du Pont de Nemours and Company
InventorsSaverio Carl Falco, Omolayo O. Famodu, Jian-Ming Lee
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a tyrosine biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the tyrosine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the tyrosine biosynthetic enzyme in a transformed host cell.
Claims14
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having chorismate mutase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:12 have at least 80% sequence identity, based on the Clustal alignment method with multiple alignment default parameters of GAP PENALTY=10 and GAP LENGTH PENALTY=10 and pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, or
- (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.