Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
AssigneeThe Trustees of Columbia University in the City of New York
InventorsJingyue Ju, Zengmin Li, Anthony Tong, James J. Russo
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Abstract
This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescence emission signature. The invention further provides for the use of said tags in multi-component analyses, including multiplex genetic analyses.
Claims3
A combinatorial fluorescence energy transfer tag which comprises the structure:
- wherein A, B, and C represent different fluorescent molecules which comprise one or more energy transfer donor and one or more energy transfer acceptor,
- wherein m and n represent any integer greater than or equal to one, and
- wherein fluorescent molecules A, B, and C are separated from each other to produce a unique fluorescence emission signature.
Dependencies are inferred from the claim text.