Antifungal assay

A method of identifying a candidate agent as a modulator of function of a tar herein said target protein comprises a protein selected from the group consisting of Schizosaccharomyces pombe Cut7;

• Saccharomyces cerevisiae CIN8;
• and Saccharomyces cerevisiae KIP1;
• or a fragment of said target protein and said method comprises:
• a) adding a candidate agent to a mixture comprising said target protein that directly or indirectly produces ADP or phosphate under conditions which normally allow the production of ADP or phosphate;
• b) subjecting the mixture to an enzymatic reaction that uses said ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized;
• and
• c) determining the level of activity of the enzymatic reaction wherein a change in said level between the presence and absence of said candidate agent indicates a modulator of said target protein function.