Inducible promoters
AssigneePioneer Hi-Bred International, Inc.
InventorsWesley B. Bruce, Carl W. Garnaat, Steven W. Ritchie
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Abstract
The present invention provides compositions and methods for regulating the expression of nucleotide sequence of interest in a plant. Compositions include a novel nucleotide sequence comprising an inducible promoter for the 5C9 gene, which encodes a maize patatin-like polypeptide. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the inducible promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in an inducible manner.
Claims27
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- b) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No. PTA-11.
A DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- b) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No, PTA-11.
A plant having stably incorporated into its genome a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- b) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No. PTA-11.
A method for regulating the expression of a nucleotide sequence of interest in a plant cell, said method comprising;
- a) stably integrating into the genome of said plant cell a DNA constrict comprising a promoter operably linked to said nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- i) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- ii) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No, PTA-11;
- and,
- b) contacting the plant cell with a stimulus, wherein said stimulus induces transcription of the nucleotide sequence of interest.
A method for regulating the expression of a nucleotide sequence of interest in a plant, said method comprising:
- a) stably integrating into the genome of a plant cell a DNA construct comprising a promoter operably linked to said nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- i) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- ii) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No. PTA-11;
- b) regenerating said cell into a plant;
- and,
- c) contacting the plant with a stimulus, wherein said stimulus induces transcription of the nucleotide sequence of interest.
A plant cell having stably integrated into its genome a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence, wherein wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- and,
- b) a nucleotide sequence comprising the plant promoter sequence contained in the plasmid deposited with the ATCC as Patent Deposit No. PTA-11.
Dependencies are inferred from the claim text.