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US 6,617,493 B1Granted
Nucleotide sequence encoding soybean vestitone reductase
AssigneeE. I. Du Pont de Nemours and Company
InventorGary Michael Fader
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding soybean enzymes that catalyze steps in biosynthesis of isoflavones, the enzyme a member selected from the group consisting of chalcone isomerase, isoflavone reductase and vestitone reductase. The invention also relates to the construction of chimeric genes encoding all or a substantial portion of the enzymes, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
Claims12
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having soybean vestitone reductase activity, wherein the nucleotide sequence encoding the polypeptide and the nucleotide sequence of SEQ ID NO:7 have at least 95% sequence identity based on the Jotun Hein alignment method;
- or
- (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.