US 6,617,492 B1 · Genetic materials for transmission into maize

Abstract

There is provided a method for transferring novel genetic materials into maize. The method for deriving these new genes is via wide cross hybrid plants produced by crossing two wild relatives of maize, Eastern gamagrass (Tripsacum) and perennial teosinte (Zea diploperennis). This invention thus relates to the novel genetic materials in hybrid seed, the hybrid plant produced by the seed and/or tissue culture, variants, mutants, modifications, and cellular and molecular components of Tripsacum-perennial teosinte hybrids, and the hybrid seed, the hybrid plant produced by the seed and/or tissue culture, variants, mutants, modifications, and cellular and molecular components of (maize X Tripsacum-perennial teosinte) and/or (Tripsacum-perennial teosinte X maize). In particular this invention is directed to the ability to transfer de novo nucleotide sequences and novel alleles from Tripsacum to maize for use in genetic analyses and selection of novel genotypes with enhanced agronomic traits such as insect and disease resistance, drought tolerance, cold tolerance, tolerance to water-logging, apomixis, totipotency, perennialism, as well as adaptation to adverse soil conditions and a carbon dioxide enriched atmosphere via (maize X Tripsacum-perennial teosinte) hybrids in corn improvement programs.

A method of screening a plant to determine whether the plant is a Tripsacum daclyloides/Zea diploperennis hybrid, said method comprising:

isolating the total genomic DNA from said plant;
digesting said genomic DNA with one to four of the restriction enzymes selected from the group consisting of EcoRI, EcoRV, HindIII and BamHI;
probing said digested genomic DNA with one or more DNA markers selected from the group consisting of the maize nuclear DNA probes, maize mitochondrial DNA probes, and Tripsacum DNA probes recited below;
and determining the presence of one or more of the following restriction fragments of the following fragment sizes, wherein said restriction fragments are characterized by the following molecular marker-restriction enzyme associations and the associated fragment sizes selected from the group consisting of:
BNL5.62, EcoRI, 10.3 kb;
npi97, HindIII, 3.9 kb;
UMC157, EcoRI, 6.5 kb and 3.3 kb;
UMC157, HindIII, 5.5 kb;
UMC157 BamHI, 14.0 kb, 8.6 kb and 4.5 kb;
UMC11, BamHI, 7.0 kb;
CSU3, BamHI, 10.0 kb, 7.6 kb and 3.5 kb;
UMC67, EcoRI, 19.2 kb;
UMC67, BamHI, 13.4 kb and 1.6 kb;
CSU92, BamHI, 13.3 kb and 7.5 kb;
62 BamHI, 12.7 kb, 9.7 kb and 6.6 kb;
UMC58, HindIII, 3.3 kb;
CSU164, EcoRI, 9.0 kb and 7.0 kb;
UMC128, HindIII, 6.0 kb;
UMC107, EcoRI, 6.3 kb and 5.3 kb;
UMC107, HindIII, 19.2 kb;
UMC140, EcoRI, 5.0 kb;
UMC140, HindIII, 6.5 kb;
UMC107, EcoRI, 6.3 kb;
adh1, HindIII, 9.4 kb;
adh1, BamHI, 9.4 kb;
UMC161, HindIII, 3.3 kb;
BNL8.29, HindIII, 8.3 kb;
UMC53, EcoRI, 9.4 kb, 3.8 kb and 3.0 kb;
UMC53, EcoRV, 8.4 kb, 3.8 kb and 3.0 kb;
UMC6, EcoRI, 3.8 kb;
UMC6, HindIII, 9.4 kb;
UMC6, BamHI, 13.2 kb, 12.7 kb, and 7.0 kb;
UMC61, HindIII, 3.4 and 2.8 kb;
UMC34, EcoRI, 7.5 kb and 5.4 kb;
UMC34, HindIII, 8.8 kb, 6.5 kb and 5.8 kb;
UMC135, HindIII, 11.6 kb and 10.8 kb;
UMC131, EcoRI, 10.6 kb, 5.8 kb and 4.3 kb;
UMC55, EcoRI, 3.9 kb;
UMC55, HindIII, 4.3 kb;
UMC5, EcoRI, 5.4 kb;
UMC5, HindIII, 6.5 kb;
UMC49, BamHI, 8.2kb, 6.0 kb, 4.2 kb, and 3.2 kb;
UMC36, BamIII, 4,2 kb;
UMC32, HindIII, 6.7 kb;
asg24, HindIII, 7.2 kb and 6.4 kb;
UMC121, EcoRI, 3.7 kb and 3.2 kb;
BNL8.35, HindIII, 9.9 kb and 8.7 kb;
UMC50, BamHI, 7.8 kb, 6.8 kb, 5.8 kb and 3.8 kb;
UMC42, HindIII, 10.4 kb, 8.9 kb, 7.6 kb, 3.7 kb and 3.0 kb;
npi247, EcoRI, 8.0 kb;
npi247, HindIII, 3.0 kb;
UMC10, HindIII, 5.9 kb and 3.0 kb;
UMC10, EcoRI, 6.5 kb and 5.5 kb;
UMC102, EcoRI, 2.7 kb;
BNL6.06, EcoRI, 6.8 kb;
BNL5.37, HindIII, 10.3 kb, 5.8 kb and 3.5 kb;
npi296, EcoRI, 7.9 kb;
UMC3, EcoRI, 2.5 kb and 2.0 kb;
npi212, HindIII, 4.3 kb;
npi212, BamHI, 5.4 kb;
UMC39, EcoRI, 12.2 kb, 9.2kb, 7.8 kb and 7.1 kb UMC63, HindIII, 9.5 kb and 4.3 kb;
UMC96, HindIII, 11.8 kb, 6.4 kb and 5.5 kb;
UMC96, BamHI, 7.5 kb;
UMC2, EcoRI, 11.8 kb, 10.4 kb and 8.0 kb;
CSU25, HindIII, 4.5 and 4.3 kb;
agrr115, EcoRI, 8.0 kb and 5.4 kb;
agrr115, BamHI, 5.4 kb and 3.5 kb, phi20725, EcoRI, 10.3 kb, 9.7 kb and 7.2 kb;
phi20725, HindIII, 1.5 kb;
UMC31, EcoRI, 5.8 kb;
UMC31, BamHI 6.5 kb;
BNL5.46, HindIII, 13.7 kb, 10.5 kb, 9.7 kb and 5.1 kb;
npi386, HindIII, 12.6 kb, 9.3 kb and 8.2 kb;
tda62, BamHI, 5.5 kb and 4.8 kb;
BNL5.71, EcoRI, 11.3 kb, 6.8 kb, and 5.7 kb;
BNL5.71, BamHI, 11.3 kb, 6.8 kb and 5.7 kb;
UMC156, HindIII, 3.0 kb;
UMC66, EcoRI, 10.5 kb and 6.5 kb;
UMC66, BamHI, 3.7 kb;
UMC19, BamHI, 12.3 kb;
UMC104, HindIII, 12.4 kb, 11.6 kb and 7.5 kb;
UMC104, BamHI, 9.4 kb;
UMC133, HindIII, 10.6 kb, 9.9 kb, 9.2 kb and 7.7 kb;
UMC52, BamHI, 8.7 kb, 6.9 kb, 3.8 kb, 3.0 kb and 2.0 kb;
BNL15.07, HindIII, 2.9 kb and 2.7 kb;
npi409, EcoRI, 9.4 kb;
npi409, HindIII, 10.4 kb, 9.0 kb and 3.9 kb;
UMC147, HindIII, 16.3 kb, 3.8 kb and 2.4 kb;
UMC90, HindIII, 7.8 kb, 2.8 kb and 2.5 kb;
UMC90, BamHI, 9.0 kb;
UMC27, HindIII, 8.3 and 4.5 kb;
tda37, BamHI, 8.2 kb and 6.5 kb;
UMC43, BamHI, 9.7 kb, 7.3 kb and 5.7 kb;
UMC40, BamHI, 7.2 kb, 4.3 kb and 4.0;
BNL7.71, HindIII, 10.6 kb;
tda62, BamIII, 5.5 kb;
UMC68, HindIII, 6.0 kb;
phi10017, BamHI, 15.1 kb and 9.5 kb;
tda50, BamHI, 8.5 kb;
npi373, HindIII, 6.5 kb, 5.6 kb and 3.0 kb;
tda204, BamHI, 4.0 kb;
npi393, EcoRI, 12.1 kb, 8.5 kb, 7,0 kb and 5.6 kb;
UMC65, HindIII, 2.9 kb;
UMC46, EcoRI, 6.5 kb and 5.6 kb;
asg7, HindIII, 6.3 kb;
UMC28, HindIII, 15.8 kb and 11.9 kb;
UMC28, BamHI, 10.0 kb, 7.6 kb and 6.6 kb;
UMC134, BamHI, 4.7 kb;
UMC134, HindIII, 7.5 kb;
asg8, HindIII, 10.8 kb, 8,7 kb and 8.4 kb;
O2, EcoRI, 9.4 kb;
BNL15.40, HindIII, 5.8 kb;
UMC116, EcoRI, 9.5 kb;
UMC110, BamHI, 10.6 kb, 4.9 kb and 3.9 kb;
BNL8.32, HindIII, 8.9 kb, 7.4 kb and 7.1 kb;
BNL14.07, EcoRI, 6.4 kb, UMC80, HindIII, 10.6 kb, 8.2 kb and 2.4 kb;
BNL16.06, EcoRI, 6.8 kb and 1.9 kb;
phi20020, HindIII, 7.8 kb and 6.6 kb;
npi114, HindIII, 10.0 kb, 8.8 kb and 6.3 kb;
BNL9.11, HindIII, 3.4 kb;
UMC103, HindIII, 6.9 kb and 5.7 kb;
UMC124, HindIII, 8.0 and 7.0;
UMC124, BamHI, 6.6 kb and 2.6 kb;
UMC120, HindIII, 3.2 kb, 2.3 kb and 1.4 kb;
UMC89, EcoRI, 7.3 kb;
UMC89, HindIII, 7.3 kb;
UMC89, BamHI, 9.5 kb, 6.0 kb, 5.2 kb and 4.5 kb;
BNL12.30, EcoRI, 3.5 kb;
UMC48, HindIII, 6.2 kb, 5.3 kb, 4.7 kb, 3.5 kb and 2.2 kb;
npi268, BamHI, 6.4 kb;
phi10005, EcoRI, 15.0 kb;
UMC113, EcoRI, 5.9 kb and 5.4 kb;
UMC113, BamHI, 12.8 kb, 11.8 kb and 10.5 kb;
UMC192, HindIII, 11.4 kb and 6.4 kb;
wx(waxy), HindIII, 21.0 kb;
CSU147, HindIII, 5.9 kb;
BNL5.10, HindIII, 6.1 kb and 4.4 kb;
UMC114, BamHI, 12.6 kb, 11.5 kb, 10.0 kb, 8.8 kb, 7.5 kb and 6.5 kb;
UMC95, EcoRI, 5.6 kb;
UMC95, HindIII, 7.7 kb, 4.8 kb and 4.1 kb;
UMC95, BamHI, 15.0 kb and 9.0 kb;
CSU61, EcoRI, 8.1 kb and 4.8 kb;
BNL7.57, EcoRI, 1.0 kb;
BNL7.57, BamHI, 11.6 kb, 5.9 kb and 1.3 kb;
CSU54, EcoRI, 14.7 kb and 12.6 kb;
phi20075, EcoRI, 7.1 kb;
npi285, EcoRI, 12.4 kb, 9.4 kb and 6.0 kb;
KSU5, EcoRI, 9.8 kb, 7.6 kb, 6.1 kb, 3.8 kb and 3.5 kb;
UMC130, EcoRI, 13.5 kb and 7.0 kb;
UMC130, HindIII, 4.8 kb and 3.2 kb;
UMC130, BamIII, 3.2 kb;
UMC152, HindIII, 12.4, 7.1 kb and 5.6 kb;
UMC64, HindIII, 3.3 kb;
phi06005, EcoRI, 12.8 kb;
UMC163, HindIII, 7.0 kb, 4.8 kb and 2.6 kb;
UMC44, HindIII, 9.8 kb, 8.7 kb, 7.2 kb, 5.5 kb and 4.0 kb;
BNL10.13, HindIII, 10.8 kb;
npi306, HindIII, 7.0 kb;
pmt1, HindIII, 2.3 kb;
pmt2, HindIII, 8.0 kb, 4.2 kb, 2.8 kb and 2.1 kb;
pmt5, HindIII, 12.3 kb, 8.1 kb, 3.6 kb, 3.2 kb and 2.5 kb;
tda48, HindIII, 8.2 kb;
tda53, HindIII, 3.8 kb and 2.2 kb;
tda168, EcoRI, 3.6 kb;
tda16, HindIII, 4.3 kb;
and tda17, HindIII, 7.0 kb.

162 more elements

Genetic materials for transmission into maize

Life of this patent

Genetic materials for transmission into maize

Life of this patent

Abstract

Claims1