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US 6,617,443 B2Granted
Method for removing endotoxins
AssigneeMerck Patent GmbH
InventorsRobertus Hendriks, Maria Wehsling, Andrea Lantos
Life of this patent
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Abstract
The invention relates to a method for removing endotoxins from nucleic acids. The endotoxins are removed by pre-incubating the nucleic acids in a salt-free detergent solution and subsequent anion exchange chromatography on a tentacle anion exchanger.
Claims20
Method for the reduction in endotoxin levels in nucleic acids originating from natural, genetic engineering or biotechnological sources, comprising the following steps:
- a) preparation of a medium which contains the nucleic acids to be purified;
- b) pre-incubation of the medium from step a) with a salt-free detergent solution;
- c) application of the incubation solution from step b) to anion-exchanger material whose functional groups are bonded to tentacles on the surface of the support;
- d) washing of the anion exchanger, in which the impurities are washed out by increasing the ion strength and/or by pH changes;
- e) elution of the sample by a further increase in the ion strength and/or by a pH change.
2 more elements
Dependencies are inferred from the claim text.