Method for recovering proteins from the interstitial fluid of plant tissues
AssigneeLarge Scale Biology Corporation
InventorsThomas H. Turpen, Stephen J. Garger, Michael J. McCulloch, Terri I. Cameron, Michelle L. Samonek-Potter, R. Barry Holtz
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Abstract
A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.
Claims30
A method for recovery of a concentrated protein or biomolecule of interest from the interstitial fluid of a plant tissue comprising the steps of:
- (a) submerging plant tissue with in buffer solution;
- (b) subjecting the plant tissue and buffer solution to a substantially vacuum environment;
- (c) separating the plant tissue from the buffer solution;
- (d) centrifuging the tissue to remove interstitial fluid;
- and
- (e) concentrating the protein or biomolecule of interest from the interstitial fluid;
- wherein said plant tissue is harvested whole tobacco leaves.
A method for recovery of a protein or biomolecule of interest from the interstitial fluid of a plant tissue comprising the steps of:
- (a) harvesting plant tissue, the plant tissue being whole tobacco leaves:
- (b) dissecting the plant leaf substantially along the midrib;
- (c) submerging the plant tissue in a buffer solution;
- (d) subjecting the plant tissue and buffer solution to a substantially vacuum environment:
- (e) separating the buffer solution from the plant tissue:
- (f) centrifuging the tissue to remove interstitial fluid:
- and
- (g) concentrating the protein or bio-molecule of interest from the interstitial fluid.
A method for recovery of a protein or bio-molecule of interest from the interstitial fluid of a plant tissue comprising the steps of:
- (a) harvesting plant tissue from a host plant, the plant tissue being whole tobacco leaves;
- (b) submerging the plant tissue in a buffer solution;
- (c) subjecting the plant tissue and buffer solution to a substantially vacuum environment;
- (d) rapidly releasing the vacuum;
- (e) separating the buffer solution from the plant tissue;
- (f) centrifuging the tissue to remove interstitial fluid;
- and
- (g) concentrating the protein or bio-molecule of interest from the interstitial fluid.
Dependencies are inferred from the claim text.